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Guide to protein purification / ed. by Richard R. Burgess; Murray P. Deutscher
Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Series: Methods in enzymology 0076-6879 ; v. 463 | Methods in enzymology ; 463Publisher: San Diego, Calif. [u.a.] : Elsevier, Acad. Press, 2009Edition: 2. edDescription: Online-Ressource (LIII, 851 S., [4] Bl.) : illustrationsISBN:- 9780123745361
- 9780123749789
- 547.7
- QP601
- QP551
- QU 55
- W1
Contents:
Summary: The second edition of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. Assembles chapters on both common and less-common relevant techniques. Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming processPPN: PPN: 1651099669Package identifier: Produktsigel: ZDB-33-EBS | ZDB-33-ESD
Why purify enzymes? / A. KornbergStrategies and considerations for protein purifications / S. Linn
Use of bioinformatics in planning a protein purification / R.R. Burgess
Preparing a purification summary table / R.R. Burgess
Setting up a laboratory / M.P. Deutscher
Buffers: principles and practice / V.S. Stoll and J.S. Blanchard
Measurement of enzyme activity / T.K. Harris and M.M. Keshwani
Quantitation of protein / J.E. Noble and M.J. Bailey
Concentration of proteins and removal of solutes / D.R. Evans, J.K. Romero and M. Westoby
Maintaining protein stability / M.P. Deutscher
Selecting an appropriate method for expressing a recombinant protein / W.H. Brondyk
Bacterial systems for production of heterologous proteins / S. Zerbs, A.M. Frank and F.R. Collart
Expression in the yeast Pichia pastoris / J.M. Cregg ... [et al.]
Baculovirus-insect cell expression systems / D.L. Jarvis
Recombinant protein production by transient gene transfer into Mammalian cells / S. Geisse and C. Fux
Tagging for protein expression / A. Malhotra
Refolding solubilized inclusion body proteins / R.R. Burgess
Advances in preparation of biological extracts for protein purification / A.C. Grabski
Isolation of subcellular organelles and structures / U. Michelsen and J. von Hagen
Protein precipitation techniques / R.R. Burgess
Affi-Gel Blue for nucleic acid removal and early enrichment of nucleotide binding proteins / M.P. Deutscher
Ion-exchange chromatography / A. Jungbauer and R. Hahn
Gel filtration / E. Stellwagen
Protein chromatography on hydroxyapatite columns / L.J. Cummings, M.A. Snyder and K. Brisack
Theory and use of hydrophobic interaction chromatography in protein purification applications / J.T. McCue
Affinity chromatography: general methods / M. Urh, D. Simpson and K. Zhao
Immobilized-metal affinity chromatography (IMAC): a review / H. Block ... [et al.]
Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography / N.E. Thompson ... [et al.]
One-dimensional gel electrophoresis / D.E. Garfin
Isoelectric focusing and two-dimensional gel electrophoresis / D.B. Friedman, S. Hoving and R. Westermeier
Protein gel staining methods: an introduction and overview / T.H. Steinberg
Elution of proteins from gels / R.R. Burgess
Performing and optimizing Western blots with an emphasis on chemiluminescent detection / A. Alegria-Schaffer, A. Lodge and K. Vattem
Detergents: an overview / D. Linke
Purification of membrane proteins / S.H. Lin and G. Guidotti
Purification of recombinant G-protein-coupled receptors / R. Grisshammer
Cell-free translation of integral membrane proteins into unilamelar liposomes / M.A. Goren ... [et al.]
Determination of protein purity / D.G. Rhodes and T.M. Laue
Determination of size, molecular weight, and presence of subunits / D.G. Rhodes, R.E. Bossio and T.M. Laue
Identification and quantification of protein posttranslational modifications / A.R. Farley and A.J. Link
Parallel methods for expression and purification / S.A. Lesley
Techniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example / A. Yan and P.J. Kiley
Rethinking your purification procedure / M.P. Deutscher
Important but little known (or forgotten) artifacts in protein biochemistry / R.R. Burgess.
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