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Imaging and spectroscopic analysis of living cells : imaging live cells in health and disease / ed. by P. Michael Conn
Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Series: Methods in enzymology 0076-6879 ; v. 506 | Methods in enzymology ; 506Publisher: Amsterdam ; Heidelberg [u.a.] : Acad. Press, 2012Edition: 1. edDescription: LII, 490 S., [16] Taf. : IllISBN:- 9780123918567
- 571.6
- QP601
Contents:
Summary: This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. The second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alikePPN: PPN: 1651274304Package identifier: Produktsigel: ZDB-33-BGM | ZDB-33-EBS | ZDB-33-ESD | BSZ-33-L1UB
Front Cover; Imaging and Spectroscopic Analysis of Living Cells: Imaging Live Cells in Health and Disease; Copyright; Contents; Contributors; Preface; Methods in Enzymology; Section One: Viruses, Microbes, and Parasites; Chapter 1 : Microbial Cells Analysis by Atomic Force Microscopy; 1. Introduction; 2. Immobilization of Microbial Cells; 2.1. Isolated cell wall; 2.2. Imaging of living cells; 2.2.1. Physical entrapment; 2.2.2. Chemical attachment; 3. Applications of AFM to Nanomicrobiology; 3.1. Imaging dynamic processes; 3.2. Elucidating of microbial cell substructures
3.3. Imaging of underlying layers4. Future Challenges; Acknowledgments; References; Chapter 2 : Understanding Parasite Transmission Through Imaging Approaches; 1. Why Imaging Parasites?; 2. In Vivo Models: ""How Much Wrong Can Be Right?""; 3. The Choice of the Appropriate Microscope; 4. New Materials Provide the Basis for New Approaches to Dissect Cell Migration; 5. Visualizing Cellular Functions in Motile Cells; 6. Applying Imaging for Drug Discovery; Acknowledgments; References; Chapter 3 : Multiphoton Microscopy Applied for Real-Time Intravital Imaging of Bacterial Infections In Vivo
1. Introduction2. Selection of Reporters for a Complex Site of Infection; 2.1. Visualizing bacteria; 2.1.1. Required materials; 2.1.2. Construction of UPEC strains with chromosomal encoding of GFP+; 2.2. Visualizing tissues; 2.2.1. Required materials; 2.2.2. Visualizing filtrate flow in the nephron; 2.2.3. Visualizing the renal vasculature; 3. Setup of a Live Imaging Platform of Infection; 3.1. Selecting and customizing a multiphoton imaging platform and analysis; 3.2. Pathogen and host; 3.2.1. Required materials; 3.2.2. Pathogen; 3.2.3. Host
3.3. Anesthesia and surgical creation of retroperitoneal window3.3.1. Required materials; 3.3.2. Presurgery preparation; 3.3.3. Surgery; 3.4. Introducing uropathogenic E. coli to initiate infection; 3.4.1. Required materials; 6.4.2. Intratubular micropuncture; 3.4.3. Imaging using the retrograde infection model of acute pyelonephritis; 4. Studying Tissue Responses in Real-Time During Infection; 4.1. Probing histological changes of the mucosal lining; 4.1.1. Required materials; 4.1.2. Integrity of the mucosal epithelium; 4.1.3. Exfoliation of epithelial cells; 4.1.4. Neutrophil Infiltration
4.2. Imaging vascular features such as rate, clotting, and integrity4.2.1. Imaging and estimation of vascular flow; 4.2.2. Imaging platelets and clots with and without anticoagulant therapy; 4.2.3. Imaging the integrity of the endothelial lining; 4.3. Local tissue oxygen tension; 4.3.1. Required materials; 4.3.2. Surgical preparation of the animal; 4.3.3. Measurement of renal PO2; 4.4. Systemic dissemination; 4.5. Imaging obstruction of a perfused environment; 5. Postimaging Analysis; 5.1. Immunohistochemistry; 5.1.1. Required materials; 6. Extending Real-Time Imaging into Molecular Analysis
6.1. Molecular view of host responses
Section 1. Viruses, microbes, and parasites. Microbial cells analysis by atomic force microscopy / David Alsteens ; Understanding parasite transmission through imaging approaches / Mirko Singer and Freddy Frischknecht ; Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo / Ferdinand X. Choong ... [et al.] ; Analysis of virus entry and cellular membrane dynamics by single particle tracking / Helge Ewers and Mario Schelhaas ; Imaging of live malaria blood stage parasites / Christof Grüring and Tobias Spielmann ; Escherichia coli K1 invasion of human brain microvascular endothelial cells / Lip Nam Loh and Theresa H. WardSection 2. Disease states. Real-time imaging after cerebral ischemia : model systems for visualization of inflammation and neuronal repair / Pierre Cordeau and Jasna Kriz ; In vivo real-time visualization of leukocytes and intracellular hydrogen peroxide levels during a zebrafish acute inflammation assay / Luke Pase, Cameron J. Nowell, and Graham J. Lieschke ; Imaging protein oligomerization in neurodegeneration using bimolecular fluorescence complementation / Federico Herrera, Susana Gonçalves, and Tiago Fleming Outeiro ; 3D/4D functional imaging of tumor-associated proteolysis : impact of microenvironment / Kamiar Moin ... [et al.] -- Section 3. Other techniques. Live cell imaging in live animals with fluorescent proteins / Robert M. Hoffman ; Protein activation dynamics in cells and tumor micro arrays assessed by time resolved Förster resonance energy transfer / Véronique Calleja, Pierre Leboucher, Banafshe ́Larijani ; Imaging stem cell differentiation for cell-based tissue repair / Zhenghong Lee ... [et al.] ; Understanding the initiation of B cell signaling through live cell imaging / Angel M. Davey ... [et al.] ; A quantitative method for measuring phototoxicity of a live cell imaging microscope / Jean-Yves Tinevez ... [et al.] ; High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines / Davide Danovi ... [et al.] ; High-throughput screening in primary neurons / Punita Sharma ... [et al.] ; Live imaging fluorescent proteins in early mouse embryos / Panagiotis Xenopoulos, Sonja Nowotschin, and Anna-Katerina Hadjantonakis ; Methods for three-dimensional analysis of dendritic spine dynamics / Enni Bertling ... [et al.] ; Imaging cell competition in Drosophila imaginal discs / Shizue Ohsawa ... [et al.] ; Live cell imaging of the oviduct / Sabine Kölle -- Computational quantification of fluorescent leukocyte numbers in zebrafish embryos / Felix Ellett and Graham J. Lieschke -- Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy / Masahiro Kitano and Takaharu Okada.
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Online-Ausg. [S.l.] : Elsevier. Online-Ressource