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Test No. 460: Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants / Organisation for Economic Co-operation and Development

Mitwirkende(r): Resource type: Ressourcentyp: Buch (Online)Buch (Online)Sprache: Englisch Reihen: OECD Guidelines for the Testing of Chemicals, Section 4Verlag: Paris : OECD Publishing, 2012Beschreibung: Online-RessourceISBN:
  • 9789264185401
Schlagwörter: Andere physische Formen: Parallelausg.: Essai n 460 ; Méthode d'essai de diffusion de fluorescéine pour identifier les substances corrosives et fortement irritantes pour l'œil DOI: DOI: 10.1787/9789264185401-enOnline-Ressourcen: Zusammenfassung: This Test Guideline describes an in vitro assay that may be used for identifying water soluble ocular corrosives and severe irritants as defined by the UN Globally Harmonized System of Classification and Labelling, Category 1. The assay is performed in a well where a confluent monolayer of Madin-Darby Canine Kidney (MDCK) is used as a separation between two chambers. It uses a fluorescein dye as marqueur. The test substance has the potential to impair the junctions of the MDCK cells and thus to increase the monolayer¡¯s permeability. Consequently the fluorescein passes through the monolayer and the fluorescein leakage (FL) increases. The FL is calculated as a percentage of leakage relative to both a blank control and a maximum leakage control. The concentration of test substance that causes 20% FL (FL20, in mg/mL) is calculated and used in the prediction model for identification of ocular corrosive and severe irritants. The cut-off value of FL20 to identify water soluble chemicals as ocular corrosives/severe irritants is ¡Ü 100mg/mL. The FL test method should be part of a tiered testing strategy.PPN: PPN: 1658117425Package identifier: Produktsigel: ZDB-13-SOC | ZDB-13-SOC-ebook
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