Atomic force microscopy for biologists / Victor J. Morris, Andrew .R Kirby, A. Patrick Gunning (Institute of Food Research, Norwich Research Park, UK )

By:

Morris, Victor J [VerfasserIn]

Contributor(s):

Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Publisher: London : Imperial College Press, [2010]Distributor: Singapore : World Scientific Publishing Co. Pte. Ltd.Copyright date: © 2010Edition: Second editionDescription: 1 Online-Ressource (XIII, 406 Seiten) : IllustrationenISBN:

9781908978219
9781848164680

Subject(s):

Genre/Form:

Lehrbuch

Additional physical formats: 9781848164673. | Erscheint auch als: Atomic force microscopy for biologists. Druck-Ausgabe 2. ed. London : Imperial College Press, 2010. XIII, 406 S.DDC classification:

570.282

RVK: RVK: UH 6310 | UH 6320Online resources:

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Summary: Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the "natural" conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems. The second edition of this bestseller has been updated to describe the latest developments in this exciting field, including a brand new chapter on force spectroscopy. The dramatic developments of AFM over the past ten years from a simple imaging tool to the multi-faceted, nano-manipulating technique that it is today are conveyed in a lively and informative narrative, which provides essential reading for students and experienced researchers alikeSummary: ch. 1. An introduction -- ch. 2. Apparatus. 2.1. The atomic force microscope. 2.2. Piezoelectric scanners. 2.3. Probes and cantilevers. 2.4. Sample holders. 2.5. Detection methods. 2.6. Control systems. 2.7. Vibration isolation : thermal and mechanical. 2.8. Calibration. 2.9. Integrated AFMs -- ch. 3. Basic principles. 3.1. Forces. 3.2. Imaging modes. 3.3. Image types. 3.4. Substrates. 3.5. Common problems. 3.6. Getting started. 3.7. Image optimisation -- ch. 4. Macromolecules. 4.1. Imaging methods. 4.2. Nucleic acids : DNA. 4.3. Nucleic acids : RNA. 4.4. Polysaccharides. 4.5. Proteins -- ch. 5. Interfacial systems. 5.1. Introduction to interfaces. 5.2. Sample preparation. 5.3. Phospholipids. 5.4. Liposomes and intact vesicles. 5.5. Lipid-protein mixed films. 5.6. Miscellaneous lipid films/surfactant films. 5.7. Interfacial protein films -- ch. 6. Ordered macromolecules. 6.1. Interfacial protein films. 6.2. Two dimensional protein crystals : an introduction. 6.3. AFM studies of 2D membrane protein crystals. 6.4. AFM studies of 2D crystals of soluble proteins -- ch. 7. Cells, tissue and biominerals. 7.1. Imaging methods. 7.2. Microbial cells : bacteria, spores and yeasts. 7.3. Blood cells. 7.4. Neurons and Glial cells. 7.5. Epithelial cells. 7.6. Non-confluent renal cells. 7.7. Endothelial cells. 7.8. Cardiocytes. 7.9. Other mammalian cells. 7.10. Plant cells. 7.11. Tissue. 7.12. Biominerals -- ch. 8. Other probe microscopes. 8.1. Overview. 8.2. Scanning tunnelling microscope (STM). 8.3. Scanning near-field optical microscope (SNOM). 8.4. Scanning ion conductance microscope (SICM). 8.5. Scanning thermal microscope (SThM). 8.6. Optical tweezers and the photonic force microscope (PPM) -- ch. 9. Force spectroscopy. 9.1. Force measurement with the AFM. 9.2. First steps in force spectroscopy: from raw data to force-distance curves. 9.3. Pulling methods. 9.4. Pushing methods. 9.5. Analysis of force-distance curves.PPN: PPN: 1659122252Package identifier: Produktsigel: ZDB-124-WOP

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