New Approaches for Flavin Catalysis / edited by Bruce A. Palfey
Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Series: Methods in enzymology ; volume 620Publisher: Cambridge, MA ; San Diego, CA ; Oxford ; London : Elsevier, Academic Press, 2019Edition: First editionDescription: 1 Online-Ressource (xx, 544 Seiten)Additional physical formats: 9780128168295 Online resources: Summary: Front Cover -- New Approaches for Flavin Catalysis -- Copyright -- Contents -- Contributors -- Preface -- Chapter One: Methods for determining the reduction potentials of flavin enzymes -- 1. Introduction -- 2. Spectroelectrochemistry method -- 3. Xanthine oxidase method -- 3.1. Materials -- 3.2. Protocol -- 3.3. Considerations -- 3.4. Data analysis -- 4. Effects of ligands -- 5. Conclusion -- References -- Chapter Two: Anaerobic methods for the transient-state study of flavoproteins: The use of specialized glassware to define ... -- 1. Introduction -- 1.1. O2 as a substrate or a reactive contaminant -- 1.2. Categories of flavoprotein reactions -- 2. Controlling the concentration of molecular oxygen -- 2.1. The limits of anaerobiosis and approach with glove-boxes -- 2.2. The advantages of specialized glassware -- 2.3. Instrument design considerations -- 2.4. The Schlenk line -- 2.5. Anaerobic vessels: Tonometers and cuvettes -- 2.6. Exchanging argon for dissolved dioxygen: Making a sample anaerobic -- 2.7. Anaerobic preparation of the rapid-mixing instrument -- 2.8. Method for mounting tonometers and syringes on the kinetic instrument -- 2.9. Gas blending and sparging to control the dioxygen concentration -- 3. Preparing reduced flavoproteins -- 3.1. Titration with sodium hydrosulfite -- 3.2. Double-mixing and reduction using the native reductant -- 3.3. Enzymatic reduction using mediators -- 3.4. Light mediated reduction -- 4. Summary and conclusion -- References -- Chapter Three: Performing anaerobic stopped-flow spectrophotometry inside of an anaerobic chamber -- 1. Introduction -- 2. Anaerobic chambers -- 2.1. Anaerobic chamber components -- 2.2. Maintaining an anaerobic chamber -- 2.2.1. Maintaining chamber anaerobiosis -- 2.2.2. Maintaining humidity and removing hydrogen sulfide -- 2.3. Flexible chambers compared to rigid chambers.PPN: PPN: 1666245321Package identifier: Produktsigel: ZDB-33-BGM | ZDB-33-ESDNo physical items for this record