Test No. 250: EASZY assay - Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos / Organisation for Economic Co-operation and Development

Contributor(s):

OECD [Herausgebendes Organ]

Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Series: OECD Guidelines for the Testing of Chemicals, Section 2Publisher: Paris : OECD Publishing, 2021Description: 1 Online-Ressource (40 p.) ; 21 x 29.7cmISBN:

9789264886100

Subject(s):

Environment

DOI: DOI: 10.1787/0a39b48b-enOnline resources:

Summary: The EASZY assay is a mechanism-based in vivo screening assay designed to detect endocrine active chemicals acting as agonist through estrogen receptors (ERs), by inducing the expression of the green fluorescent protein (GFP) driven by the cyp19a1b promoter. The EASZY assay allows for the detection of estrogenic activity of chemicals on transgenic tg(cyp19a1b:GFP) Zebrafish embryos exposed for 96 hours during the embryonic stages of development. At the end of the experiment, the fluorescence of each newly hatched eleutheroembryo is measured using fluorescence microscope. Because the skull of early developmental stages of zebrafish is transparent, GFP is observed, imaged and quantified in vivo. The intensity of fluorescence is then quantified using image analysis software.Other editions: Parallele Sprachausgabe: Essai n° 250: Détection sur des embryons de poisson-zèbre transgénique tg(cyp19a1b:GFP) des perturbateurs endocriniens agissant via les récepteurs des œstrogènes (essai EASZY)PPN: PPN: 1770755691Package identifier: Produktsigel: ZDB-13-SOC | ZDB-13-SOC-ebook

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