Fluorescent dye labels and stains : a database of photophysical properties / Tarso B Ledur Kist

By: Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Publisher: Hoboken, NJ : John Wiley & Sons, 2023Description: 1 Online-Ressource (1 online resource)Subject(s): Additional physical formats: 9781119835165. | 111983516X | 9781119835141. | 1119835143 | 9781119835158. | 1119835151 | 9781119835134. | 9781119835134 | Erscheint auch als: Fluorescent dye labels and stains. Druck-Ausgabe Hoboken, NJ : John Wiley & Sons, 2023DDC classification:
  • 570.28/2
LOC classification:
  • QH212.F55
Online resources: Summary: "This book presents the molecular structures and photophysical properties of over seven-hundred fluorescent dyes with medium to high brightness in aqueous solutions. It has been written to be a reliable and useful source of information for users of fluorescent dye labels and stains in general. The specific aqueous solution used to measure the photophysical parameters of each of the seven-hundred dyes is clearly provided in this succinct database. The photophysical parameters presented in this database include: wavelength of absorption and/or excitation maximum, wavelength of emission maximum, Stokes shifts, excitation range, emission range, molar absorption coefficients, fluorescence quantum yields, brightness, fluorescence mean-lifetimes, sizes (represented by the number of atoms of the structures), and charge in aqueous solutions at neutral pH. Additional interesting properties of some of the fluorescent dyes have also been included, such as photostability and pKa (when present at around neutral pH). Scattergrams are systematically used to give panoramic views of the photophysical data that allow users to find desired photophysical properties within a given spectral range. Previously, this data was only available from varied sources that contained partial lists, were missing data, and, in many cases, omitted descriptions of the liquids in which parameters were measured. It is known that almost all fluorescent dyes that exhibit high molecular fluorescence brightness in aqueous solutions are also bright in organic solvents; however, the opposite is not true. Most dyes that exhibit a high brightness in organic solvents exhibit a lower and, in many cases, a very low brightness in aqueous solutions. Even worse, the great majority of fluorescent dyes available are not soluble enough in aqueous solutions to have their photophysical parameters measured. Hence the importance of this database, where only dyes whose photophysical parameters were measured in plain water, phosphate-buffered saline, or aqueous buffers (without organic modifiers) are included. Some dyes are not commercially available anymore, but were nonetheless kept in the database for completeness and historic record purposes"--PPN: PPN: 188263408XPackage identifier: Produktsigel: ZDB-4-NLEBK | BSZ-4-NLEBK-KAUB
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