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Live cell imaging : a laboratory manual / ed. by Robert D. Goldman; David L. Spector
Contributor(s): Resource type: Ressourcentyp: BuchBookLanguage: English Publisher: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, 2005Description: XVI, 631 S. : Ill., graph. Darst. ; 29 cm. + 1 DVDISBN:- 0879696834
- 0879696826
- 571.6078
- 571.6/078
- QH583.2
- QH583.2 .L485 2005
- QH583.2.L485 2004
- QH 583.2
Contents:
Summary: Sect. 1. Detection and approaches to live cell imaging. Fluorescent protein tracking and detection -- Constructing and expressing GFP fusion proteins -- Viral vectors for introduction of GPF -- Gene delivery by direct injection and facilitation of expression by mechanical stretch -- Microinjection of fluorophore-labeled proteins -- CCD cameras for fluorescence imaging of living cells -- Photobleaching techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, iFRAP, and FLIP -- FRET and fluorescence lifetime imaging microscopy -- Monitoring protein dynamics using FRET-based biosensors -- Application of light-directed activation of caged biomolecules and CALI to problems in cell motility -- Photoactivation-based labeling and in vivo tracking of RNA molecules in the nucleus -- Fluorescent speckle microscopy of cytoskeletal dynamics in living cells -- Polarization microscopy with the LC-PolScope -- Confocal microscopy, deconvolution, and structured illumination methods -- Multiphoton and multispectral laser-scanning microscopy -- Analyzing live cell data and tracking dynamic movements --Summary: Sect. 2. Imaging of live cells and organisms. In vivo imaging of mammalian cells -- A sealed preparation for long-term observations of cultured cells -- Live cell imaging of yeast -- Live imaging of Caenorhabditis elegans -- Time-lapse cinematography in living Drosophila tissues -- Single-cell imaging in animal tumors in vivo -- Long-term, high-resolution imaging in the neocortex in vivo -- Intravital microscopy of normal and diseased tissues in the mouse -- Development of mammalian cell lines with lac operator-tagged chromosomes -- Imaging gene expression in living cells -- Studying mitosis in cultured mammalian cells -- Imaging Hoechst-33342-labeled chromosomes and fluorescent proteins during the cell cycle -- Imaging the actin cytoskeleton -- Imaging intermediate filament proteins in living cells -- Methods for expressing and analyzing GFP-tubulin and GFP-microtubule-associated proteins -- Imaging of organelle membrane systems and membrane traffic in living cells -- Imaging live cells under mechanical stress -- Imaging single molecules using total internal reflection fluorescence microscopy -- Visualization and quantification of single RNA molecules in living cellsCall number: Grundsignatur: 2008 E 514PPN: PPN: 378274589
| Item type | Home library | Collection | Shelving location | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|---|
| Freihandbestand ausleihbar | Bibliothek Campus Süd | nat 2.11 | Lesesaal Naturwissenschaften (LSN) | 2008 E 514 | Available | 49276252090 | ||||
| Freihandbestand Präsenznutzung | Bibliothek Campus Süd | nat 2.11 | Lesesaal Naturwissenschaften (LSN) | 2008 E 514 | ;b | Not for loan | 49276218090 | |||
| Freihandbestand Präsenznutzung | Fachbibliothek Physik | nat 2.11 | Bibliothek / frei aufgestellt | 2008 E 514 | ;c | Not for loan | 49276172090 | |||
| Freihandbestand Präsenznutzung | Fachbibliothek Physik | nat 2.11 | Bibliothek / frei aufgestellt | 2008 E 514 | ;d | Not for loan | 49276650090 |
Total holds: 0