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Test No. 455: The Stably Transfected Human Estrogen Receptor-alpha Transcriptional Activation Assay for Detection of Estrogenic Agonist-Activity of Chemicals / Organisation for Economic Co-operation and Development

Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Series: OECD Guidelines for the Testing of Chemicals, Section 4Publisher: Paris : OECD Publishing, 2009Description: Online-Ressource (16 p)ISBN:
  • 9789264076372
  • 9789264076372
Subject(s): Additional physical formats: Parallelausg.: Essai n 455 ; Essai d'activation transcriptionnelle faisant intervenir le récepteur d'œstrogène alpha humain transfecté de façon stable pour la détection de l'activité œstrogénique agoniste des substances testées | Parallelausg.: Essai n 455 : Essai d'activation transcriptionnelle faisant intervenir le récepteur d'œstrogène alpha humain transfecté de façon stable pour la détection de l'activité œstrogénique agoniste des substances testées DOI: DOI: 10.1787/9789264076372-enOnline resources: Summary: This Test Guideline describes an in vitro assay, which provides mechanistical information, and can be used for screening and prioritization purposes. The test system utilises the hERalpha-HeLa-9903 cell line derived from a human cervical tumor and stably transfected. This cell line can measure the ability of a test chemical to induce hERalpha-mediated transactivation of luciferase gene expression. The cells are exposed to 7 non-cytotoxic concentrations of the test chemical for 20-24 hours to induce the reporter gene products. Four reference chemicals should be included in each experiment: a strong estrogen (17beta-estradiol), a weak estrogen (17alpha-estradiol), a very weak estrogen (17alpha-methyltestosterone) and a negative control (corticosterone). The activity of the luciferase enzyme is measured in a luminometer. A test chemical is considered to be positive if the maximum response induced is equal to or exceeds 10% of the response of the positive control (1 nM 17alpha-estradiol) in at least two of two or two of three runs. Software to be used with TG 425, 432, 455. Click here. Software not part of the Mutual Acceptance of Data.PPN: PPN: 723693358Package identifier: Produktsigel: ZDB-13-SOC | ZDB-13-SOC-ebook
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