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Immunoassays in agricultural biotechnology / edited by Guomin Shan
Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Publisher: Hoboken, New Jersey : Wiley, [2011], (c)2011Edition: Online-AusgDescription: Online-Ressource (1 online resource (xii, 650 p.)) : illISBN:- 9780470909928
- 9781283100458
- 1283100452
- 616.0756
- 616.07/56 22
- SCI013010
- QP519.9.I42
Contents:
Summary: A comprehensive how-to guide on using immunoassay technology with genetically modified crops With the dramatic rise in the adoption of genetically engineered (GE) or genetically modified (GM) crops in countries around the world, scientists and professionals in government, academia, and agriculture are increasingly required to know how to use immunochemistry technology to detect and monitor GM plants and related products. Immunoassays in Agricultural Biotechnology provides for the first time a comprehensive, easy-to-use technical guide on the application of immunoassay methods for the analysis of GM crops and related areas. Geared to readers with varying degrees of technical skill and experience in the field, this handy book explains topics from the ground up. It also includes methods and protocols, essential reference materials, and much more. Contributed chapters by international experts cover a range of topics, from current technologies to food regulation, including: All aspects of assay development, including formats, kit development, manufacturing, and quality control Immunoassay method validation techniques Applications in GM crop research, product development, and commercialization Immunoassay automation and high throughput in plant sample analysis The latest applications of immunoassays in seed, grain, and food processing Environmental applications and soil monitoring Applications in animal vaccine development and biopharmaceutical research Regulatory issues, including trade barriers and the negative aspects of GM technology.Summary: IMMUNOASSAYS IN AGRICULTURAL BIOTECHNOLOGY -- CONTENTS -- FOREWORD -- PREFACE -- CONTRIBUTORS -- 1 INTRODUCTION -- 2 PRINCIPLES OF IMMUNOASSAYS -- 3 ANTIBODY ENGINEERING IN AGRICULTURAL BIOTECHNOLOGY -- 4 MICROTITER PLATE ELISA -- 5 LATERAL FLOW DEVICES -- 6 IMMUNOASSAY METHOD VALIDATION -- 7 REFERENCE MATERIALS AND CONSIDERATIONS -- 8 AUTOMATION OF IMMUNOASSAYS -- 9 DATA INTERPRETATION AND SOURCES OF ERROR -- 10 IMMUNOASSAY APPLICATIONS IN TRAIT DISCOVERY, PRODUCT DEVELOPMENT, AND REGISTRATION -- 11 IMMUNOASSAY APPLICATIONS IN GRAIN PRODUCTS AND FOOD PROCESSING -- 12 IMMUNOASSAY APPLICATIONS IN SOIL MONITORING -- 13 IMMUNOASSAY APPLICATIONS IN PLANT-BASED BIOPHARMA -- 14 IMMUNOASSAYS IN VETERINARY PLANT-MADE VACCINES -- 15 IMMUNOASSAY AS A GM DETECTION METHOD IN INTERNATIONAL TRADE -- 16 FUTURE PERSPECTIVES AND CHALLENGES -- INDEX.PPN: PPN: 80733569XPackage identifier: Produktsigel: ZDB-26-MYL | ZDB-30-PAD | ZDB-30-PQE
""Title Page""; ""Copyright""; ""Foreword""; ""Preface""; ""Contributors""; ""Chapter 1: Introduction""; ""References""; ""Chapter 2: Principles of Immunoassays""; ""2.1 Introduction""; ""2.2 Antigens""; ""2.3 Antibodies""; ""2.4 Antibody Development and Production""; ""2.5 Antibodyâ€"Antigen Interactions""; ""2.6 Conclusion""; ""References""; ""Chapter 3: Antibody Engineering in Agricultural Biotechnology""; ""3.1 Introduction""; ""3.2 Recombinant Antibody Fragments""; ""3.3 Development and Types of Antibody Libraries""; ""3.4 Display Systems""; ""3.5 Optimization of Recombinant Antibodies""
""3.6 Expression and Purification""""3.7 Application to Immunoassays and Agricultural Biotechnology""; ""References""; ""Chapter 4: Microtiter Plate Elisa""; ""4.1 ELISA: A Historical View""; ""4.2 Assay Design Considerations""; ""4.3 Assay Optimization""; ""4.4 Formats for ELISA""; ""4.5 Further Performance Optimization""; ""4.6 Troubleshooting""; ""4.7 Future Applications of Microtiter Plate ELISA""; ""References""; ""Chapter 5: Lateral Flow Devices""; ""5.1 Introduction""; ""5.2 Principle of Lateral Flow Devices""; ""5.3 Advantages and Limitations""; ""5.4 Development and Validation""
""5.5 Application and Troubleshooting""""References""; ""Chapter 6: Immunoassay Method Validation""; ""6.1 Introduction""; ""6.2 Quantitative ELISA Validation""; ""6.3 Independent Laboratory Validations""; ""6.4 Western Blot Method Validation""; ""6.5 Qualitative Immunoassay Validations""; ""6.6 GIPSA Performance Verification of Rapid Test Kits""; ""6.7 Validation Results""; ""References""; ""Chapter 7: Reference Materials and Considerations""; ""7.1 General Considerations""; ""7.2 Sources and Availability of Protein Reference Materials""
""7.3 Role of Reference Materials and Immunoassays for Testing Genetically Engineered Traits""""7.4 Quality and Performance Characteristics of Protein-Based Reference Materials""; ""7.5 Harmonizing Protein with DNA Certified Reference Materials""; ""7.6 Applications and Limitations of Protein-Based Reference Materials""; ""7.7 Challenges and Future Needs""; ""References""; ""Chapter 8: Automation of Immunoassays""; ""8.1 Introduction to Immunoassay Automation""; ""8.2 Increasing Throughput by Automation""; ""8.3 Tissue Collection""; ""8.4 Sample Processing""; ""8.5 Sample Transfer""
""8.6 Reagent Additions""""8.7 Washing and Reading Steps""; ""8.8 Analysis""; ""8.9 Achieving Full Automation by Connecting the Automated Steps""; ""8.10 Duplexing""; ""8.11 Future Technologies""; ""8.12 Summary""; ""References""; ""Chapter 9: Data Interpretation and Sources of Error""; ""9.1 Introduction""; ""9.2 Data Interpretation""; ""9.3 Sources of Error""; ""References""; ""Chapter 10: Immunoassay Applications in Trait Discovery, Product Development, and Registration""; ""10.1 Introduction""; ""10.2 Discovery, Transformation, and Event Selection""; ""10.3 Breeding and Backcrossing""
""10.4 Role of Immunoassays in Registration""
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