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Kinetics of enzyme action : essential principles for drug hunters / Ross L. Stein

By: Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Publisher: Hoboken, N.J : John Wiley, c2011Edition: Online-AusgDescription: Online-Ressource (1 online resource (xii, 302 p.)) : illISBN:
  • 9781283176217
  • 1283176211
  • 9781118084397
Subject(s): Additional physical formats: 9780470414118 | 9781118084397 | 0470414111 | 1283174774 | Druckausg.: Kinetics of enzyme action. Hoboken, NJ : Wiley, 2011. XII, 302 S.DDC classification:
  • 572.7
  • 572/.7 23
RVK: RVK: VK 8700LOC classification:
  • QP601
NLM classification:
  • QU 135
Online resources: Summary: "Dr. Stein . . . make[s] a difficult topic easy to understand and comprehend." -Richard Harrison, Wyeth Research Details on the approaches and applications essential to kinetically characterizing enzyme inhibitors for drug discovery The ability of enzymes to bring about the transformation of one chemical substance into another makes them the favorite-and most productive-drug target in the pharmaceutical industry. Kinetics of Enzyme Action explores, in depth, the characteristics and applications of these proteins and their vital role in the development of new medicines by placing the subject of enzymology in a historical context that enriches and heightens understanding of this complex science. It introduces readers to a wide range of topics invaluable for helping them discover and kinetically characterize enzyme inhibitors-from simple, one-substrate enzyme reactions to the more complex two-substrate enzymes reactions. Some of the highlights in this book include: A detailed description of enzyme kinetics as applied to drug discovery A comprehensive collection of theory and pragmatic approaches to guide readers in the science of performing detailed enzyme mechanistic studies An outline of the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery Numerous case studies from scientific literature that bring the topics to life From discussion of the early developments in the kinetics of enzymatic reactions to fresh insights on contemporary enzymology, Kinetics of Enzyme Action offers comprehensive coverage of a discipline that holds vital significance for all pharmaceutical practitioners.Summary: Intro -- KINETICS OF ENZYME ACTION: Essential Principles for Drug Hunters -- CONTENTS -- PREFACE -- 1: INTRODUCTION -- 1.1 A BRIEF HISTORY OF ENZYMOLOGY -- 1.2 GOAL OF ENZYMOLOGY: THE ELUCIDATION OF MECHANISM -- 1.3 THE EMERGENCE OF MECHANISM FROM DATA -- 2: KINETICS OF SINGLE-SUBSTRATE ENZYMATIC REACTIONS -- 2.1 THE DEPENDENCE OF INITIAL VELOCITY ON SUBSTRATE CONCENTRATION AND THE REQUIREMENT FOR AN E : S COMPLEX -- 2.2 DERIVATION OF THE RATE EQUATION FOR A SINGLE-SUBSTRATE REACTION WITH THE ASSUMPTION OF RAPID EQUILIBRIUM -- 2.3 DERIVATION OF RATE EQUATIONS USING THE STEADY-STATE ASSUMPTION -- 2.4 METHODS OF ENZYME ASSAY -- 2.5 ENZYME KINETICS PRACTICUM: ASSAY DEVELOPMENT, EXPERIMENTAL DESIGN, DATA COLLECTION, AND DATA ANALYSIS -- 3: KINETICS OF SINGLE-SUBSTRATE ENZYMATIC REACTIONS: SPECIAL TOPICS -- 3.1 TRANSITION STATE THEORY AND FREE ENERGY DIAGRAMS -- 3.2 KINETIC CONSEQUENCES OF AN ENZYME : SUBSTRATE COMPLEX -- 3.3 REACTIONS WITH MORE THAN ONE INTERMEDIARY COMPLEX -- 3.4 DEVIATIONS FROM MICHAELIS-MENTEN KINETICS -- 3.5 KINETICS OF ENZYMATIC ACTION ON SUBSTRATES WITH MULTIPLE REACTIVE CENTERS -- 4: ENZYME INHIBITION: THE PHENOMENON AND MECHANISM-INDEPENDENT ANALYSIS -- 4.1 ENZYME INHIBITION: THE PHENOMENON -- 4.2 ENZYME INHIBITION: THE FIRST QUANTITATIVE STEPS -- 4.3 ENZYME-INHIBITOR SYSTEMS MISBEHAVING -- 4.4 CASE STUDIES -- 5: KINETIC MECHANISM OF INHIBITION OF ONE-SUBSTRATE ENZYMATIC REACTIONS -- 5.1 IMPORTANCE IN DRUG DISCOVERY -- 5.2 THEORETICAL CONSIDERATIONS -- 5.3 ANALYSIS OF INITIAL VELOCITY DATA FOR ENZYME INHIBITION -- 5.4 INHIBITION OF ONE-SUBSTRATE, TWO-INTERMEDIATE REACTIONS -- 5.5 INHIBITION BY DEPLETION OF SUBSTRATE -- 6: TIGHT-BINDING, SLOW-BINDING, AND IRREVERSIBLE INHIBITION -- 6.1 IMPORTANCE IN DRUG DISCOVERY -- 6.2 TIGHT-BINDING INHIBITION -- 6.3 SLOW-BINDING INHIBITION -- 6.4 IRREVERSIBLE INHIBITION.PPN: PPN: 807339938Package identifier: Produktsigel: ZDB-26-MYL | ZDB-30-PAD | ZDB-30-PQE
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