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Test No. 476: In Vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes / Organisation for Economic Co-operation and Development

Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Series: OECD Guidelines for the Testing of Chemicals, Section 4Publisher: Paris : OECD Publishing, 2015Description: Online-Ressource (18 p.)ISBN:
  • 9789264243088
Subject(s): Additional physical formats: Parallelausg.: Essai n 476 ; Essais in vitro de mutation génique sur cellules de mammifères utilisant les gènes Hprt et xprt | Parallelausg.: Essai n 476: Essais in vitro de mutation génique sur cellules de mammifères utilisant les gènes Hprt et xprt DOI: DOI: 10.1787/9789264243088-enOnline resources: Summary: The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. In this test, the used genetic endpoints measure mutation at hypoxanthine-guanine phosphoribosyl transferase (HPRT), and at a transgene of xanthineguanine phosphoribosyl transferase (XPRT). The HPRT and XPRT mutation tests detect different spectra of genetic events. Cells in suspension or monolayer culture are exposed to, at least four analysable concentrations of the test substance, both with and without metabolic activation, for a suitable period of time. They are subcultured to determine cytotoxicity and to allow phenotypic expression prior to mutant selection. Cytotoxicity is usually determined by measuring the relative cloning efficiency (survival) or relative total growth of the cultures after the treatment period. The treated cultures are maintained in growth medium for a sufficient period of time, characteristic of each selected locus and cell type, to allow near-optimal phenotypic expression of induced mutations. Mutant frequency is determined by seeding known numbers of cells in medium containing the selective agent to detect mutant cells, and in medium without selective agent to determine the cloning efficiency (viability). After a suitable incubation time, colonies are counted.PPN: PPN: 838080278Package identifier: Produktsigel: ZDB-13-SOC | ZDB-13-SOC-ebook
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