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Single molecule biophysics and poisson process approach to statistical mechanics / Susanta K. Sarkar (Colorado School of Mines, USA)

By: Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Series: IOP concise physicsPublisher: San Rafael, CA : Morgan & Claypool Publishers, [2016]Distributor: Bristol : IOP PublishingEdition: Version: 20160401Description: 1 Online-Ressource (75 Seiten in getrennter Zählung) : Illustrationen, DiagrammeISBN:
  • 9781681741161
  • 9781681742441
  • 1681741164
  • 1681742446
Subject(s): Additional physical formats: 9781681740522 | Erscheint auch als: 9781681740522 Druck-AusgabeDDC classification:
  • 571.4
LOC classification:
  • QH505
NLM classification:
  • QT 34
DOI: DOI: 10.1088/978-1-6817-4116-1Online resources: Additional physical formats: Also available in print.Summary: This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of scienceSummary: Preface -- 1. Single molecule biophysics -- 1.1. Introduction -- 1.2. A brief history of single molecule study -- 1.3. Single molecule methods -- 1.4. Single molecule data analysis -- 1.5. Brief overview of thermodynamics and statistical mechanics -- 1.6. Future outlook -- 1.7. ConclusionSummary: 2. The total internal reflection fluorescence microscope -- 2.1. Introduction -- 2.2. Design considerations -- 2.3. Microscope frame and associated parts -- 2.4. Microscope objectives -- 2.5. Detection path and camera -- 2.6. Excitation path -- 2.7. Computer, computer software, and other computing hardware -- 2.8. Experimental protocol for slide preparation -- 2.9. Specifications of the TIRF microscope -- 2.10. Objective-type TIRFM -- 2.11. Multichannel detection and FRET -- 2.12. Circular dichroism spectroscopy and microscope enclosure -- 2.13. AcknowledgmentsSummary: 3. Poisson process approach to statistical mechanics -- 3.1. Introduction -- 3.2. Diffusion -- 3.3. Inter-conversion of the states of molecules -- 3.4. Photon statistics from lasers -- 3.5. Statistics of fluorescent centers on a surface -- 3.6. Location of stars in the sky -- 3.7. Quantifying the heterogeneity of the locations of cosmic ray sources -- 3.8. Conclusion -- 3.9. AcknowledgmentsSummary: Appendices. -- A. Building a total internal reflection fluorescence microscope: parts, vendors, and costs -- B. The Poisson processPPN: PPN: 858960117Package identifier: Produktsigel: ZDB-135-ICP | ZDB-135-IAL
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