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Principles of three dimensional imaging in confocal microscopes / Min Gu

By: Contributor(s): Resource type: Ressourcentyp: Buch (Online)Book (Online)Language: English Publisher: Singapore ; River Edge, N.J : World Scientific Pub. Co, 1996Description: 1 Online-Ressource (xii, 337 p) : illISBN:
  • 9810225504
  • 9789814261104
  • 9789810225506
Subject(s): Additional physical formats: Erscheint auch als: 9810225504 Druck-Ausgabe | Erscheint auch als: 9789810225506 Druck-AusgabeDDC classification:
  • 681.413 22
LOC classification:
  • QH224
DOI: DOI: 10.1142/3014Online resources: Additional physical formats: Electronic reproduction; System requirements: Adobe Acrobat ReaderSummary: 1. Introduction. 1.1. Confocal microscopy. 1.2. Coherence of confocal microscopy. 1.3. Previous studies on three-dimensional optical microscopy. 1.4. Concept of transfer functions. 1.5. Overview -- 2. Three-dimensional fourier optics of a thin lens. 2.1. Diffraction of a thin lens. 2.2. Three-dimensional coherent image formation. 2.3. Three-dimensional space-invariant point spread function. 2.4. Three-dimensional coherent transfer function. 2.5. Three-dimensional optical transfer function. 2.6. Three-dimensional imaging in a conventional microscope -- 3. Confocal bright-field microscopy with a point detector. 3.1. Coherent image formation. 3.2. Coherent transfer function. 3.3. Effects of spherical aberration. 3.4. Imaging with detector offset. 3.5. Influence of pupil functions -- 4. Confocal bright-field microscopy with a finite-sized detector. 4.1. Partially-coherent image formation. 4.2. Intensity point spread function. 4.3. Transmission cross-coefficient. 4.4. Effect of detector size on axial resolution. 4.5. Effect of detector size on transverse resolution - Edge-setting criteria. 4.6. Weak-object transfer function -- 5. Confocal single-photon fluorescence microscopy. 5.1. Incoherent image formation. 5.2. Optical transfer function. 5.3. Effect of annular pupils on optical transfer functions. 5.4. Signal level. 5.5. Improvement in axial resolution. 5.6. Effect of finite-sized source -- 6. Confocal two-photon fluorescence microscopy. 6.1. Incoherent image formation. 6.2. Intensity point spread function. 6.3. Optical transfer function. 6.4. Comparison of resolution in two-photon and single-photon fluorescence microscopy. 6.5. Signal level -- 7. Fibre-optical confocal microscopy. 7.1. Fibre-optical confocal scanning microscopy. 7.2. Bright-field imaging. 7.3. Axial resolution. 7.4. Transverse resolution - Image of a straight edge. 7.5. Signal level. 7.6. Fibre-optical confocal interferometry. 7.7. Fluorescence imaging -- 8. Confocal microscopy under ultrashort pulse illumination. 8.1. Fourier optics for a thin lens under ultrashort pulse illumination. 8.2. Bright-field imaging. 8.3. Fluorescence imaging -- 9. Confocal microscopy with high-aperture objectives. 9.1. Confocal bright-field imaging. 9.2. Confocal fluorescence imaging. 9.3. Confocal 4Pi imaging -- 10. Significance of three-dimensional transfer functions. 10.1. Imaging of thick planar layers. 10.2. Imaging of a thin object. 10.3. Imaging of a line object. 10.4. Imaging of a point object. 10.5. Imaging with the extended-focus technique. 10.6. Summary -- Hankel transform.Summary: This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functionsPPN: PPN: 877513511Package identifier: Produktsigel: ZDB-124-WOP
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Electronic reproduction; System requirements: Adobe Acrobat Reader

Reproduktion. Singapore : World Scientific Publishing Co. Electronic reproduction; System requirements: Adobe Acrobat Reader

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