A structure-function toolbox for membrane transporter and channels / edited by Christine Ziegler

Mitwirkende(r):

Ziegler, Christine [HerausgeberIn]

Resource type: Ressourcentyp: Buch (Online)Buch (Online)Sprache: Englisch Reihen: Methods in enzymology ; volume 594Verlag: Cambridge, MA ; San Diego, CA ; Kidlington, Oxford ; London : Academic Press, [2017]Copyright-Datum: ©2017Auflage: First editionBeschreibung: 1 Online-Ressource (xii, 289 Seiten)ISBN:

9780128123539
9780128123546
9780128123539

Schlagwörter:

Membrantransport

Genre/Form:

Aufsatzsammlung

Andere physische Formen: 9780128123539 | Erscheint auch als: A structure-function toolbox for membrane transporter and channels. Druck-Ausgabe. First edition. Cambridge, MA : Academic Press, an imprint of Elsevier, 2017. xii, 289 Seiten | Print version: A Structure-Function Toolbox for Membrane Transporter and Channels. Saint Louis : Elsevier Science,c2017RVK: RVK: WC 4355Online-Ressourcen:

Zugang im Netz des KIT

Zusammenfassung: Title page -- Table of Contents -- Copyright -- Contributors -- Preface -- Chapter One: Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM -- Abstract -- 1 Introduction -- 2 General Considerations -- 3 Single-Particle Cryo-EM of the Ryanodine Receptor RyR1 Reconstituted Into Lipid Nanodiscs -- 4 EM Analysis of the TcdA1 Pore Complex Embedded in Lipid Nanodiscs -- 5 Conclusions and Perspectives -- Acknowledgments -- Chapter Two: SSM-Based Electrophysiology for Transporter Research -- Abstract -- 1 Background -- 2 Technical Implications -- 3 Description of the Workflow -- 4 Assay Development -- 5 Data Interpretation and Analysis -- Chapter Three: Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters -- Abstract -- 1 Introduction -- 2 Membrane Protein Reconstitution -- 3 EM of Salipro Particles -- 4 Summary and Conclusion -- Acknowledgments -- Chapter Four: Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy -- Abstract -- 1 Introduction -- 2 Preparing Membrane Proteins and Lipid Membranes -- 3 Single-Molecule Fluorescence Microscopy -- 4 Data Analysis -- Acknowledgments -- Chapter Five: The MEMbrane Protein Single ShoT Amplification Recipe: MemStar -- Abstract -- 1 Introduction -- 2 Materials -- 3 Procedure -- 4 Summary and Conclusion -- Acknowledgments -- Chapter Six: Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli -- Abstract -- 1 Introduction -- 2 Preparation of Expression Vectors and Cloning -- 3 Analysis of Membrane Protein Production Using GFP -- 4 Summary and Conclusion -- Acknowledgments -- Chapter Seven: Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific LabelingZusammenfassung: Abstract -- 1 Introduction -- 2 The Unique and Useful Chemistry of Cysteine -- 3 The Many Uses of Cysteine -- 4 Cysteine-Reactive Reagents Used for Cross-Linking and Labeling -- 5 Methods of Detecting Cross-Link Formation -- 6 Strategies Used to Decide the Position of Cysteine Residues -- 7 Detailed Protocol for Cross-Linking a Transporter -- Chapter Eight: Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR -- Abstract -- 1 Introduction -- 2 Designing the Pulsed-EPR Experiment -- 3 Sample Preparation for EPR Experiments -- 4 The EPR Methodology Toolbox -- Acknowledgments -- Chapter Nine: Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein -- Abstract -- 1 Introduction -- 2 A Thermostability Assay for Measuring the Stability of GPCR-G Protein Complexes -- 3 Summary and Conclusion -- Acknowledgments -- Author Index -- Subject IndexPPN: PPN: 895898217Package identifier: Produktsigel: H-ZDB-33-BGM | H-ZDB-33-ESD

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